Witman Lab Publications

Title

Isolation of Chlamydomonas flagella

UMMS Affiliation

Department of Cell and Developmental Biology

Publication Date

6-1-2013

Document Type

Book Chapter

Subjects

Chlamydomonas; Flagella

Disciplines

Cell and Developmental Biology | Cell Biology | Laboratory and Basic Science Research

Abstract

A simple, scalable, and fast procedure for the isolation of Chlamydomonas flagella is described. Chlamydomonas can be synchronously deflagellated by treatment with chemicals, pH shock, or mechanical shear. The Basic Protocol describes the procedure for flagellar isolation using dibucaine to induce flagellar abscission; we also describe the pH shock method as an Alternate Protocol when flagellar regeneration is desirable. Sub-fractionation of the isolated flagella into axonemes and the membrane + matrix fraction is described in a Support Protocol.

DOI of Published Version

10.1002/0471143030.cb0341s59

Source

Craige, B., Brown, J. M. and Witman, G. B. 2013. Isolation of Chlamydomonas Flagella. Current Protocols in Cell Biology. 2013 Jun;Chapter 3:Unit 3.41.1-9. doi: 10.1002/0471143030.cb0341s59. Link to article on publisher's website

Journal/Book/Conference Title

Current Protocols in Cell Biology

Related Resources

Link to article in PubMed

PubMed ID

23728744

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