Witman Lab Publications
Title
Isolation of Chlamydomonas flagella
UMMS Affiliation
Department of Cell and Developmental Biology
Publication Date
6-1-2013
Document Type
Book Chapter
Subjects
Chlamydomonas; Flagella
Disciplines
Cell and Developmental Biology | Cell Biology | Laboratory and Basic Science Research
Abstract
A simple, scalable, and fast procedure for the isolation of Chlamydomonas flagella is described. Chlamydomonas can be synchronously deflagellated by treatment with chemicals, pH shock, or mechanical shear. The Basic Protocol describes the procedure for flagellar isolation using dibucaine to induce flagellar abscission; we also describe the pH shock method as an Alternate Protocol when flagellar regeneration is desirable. Sub-fractionation of the isolated flagella into axonemes and the membrane + matrix fraction is described in a Support Protocol.
DOI of Published Version
10.1002/0471143030.cb0341s59
Source
Craige, B., Brown, J. M. and Witman, G. B. 2013. Isolation of Chlamydomonas Flagella. Current Protocols in Cell Biology. 2013 Jun;Chapter 3:Unit 3.41.1-9. doi: 10.1002/0471143030.cb0341s59. Link to article on publisher's website
Journal/Book/Conference Title
Current Protocols in Cell Biology
Related Resources
PubMed ID
23728744
Repository Citation
Craige, Branch; Brown, Jason M.; and Witman, George B., "Isolation of Chlamydomonas flagella" (2013). Witman Lab Publications. 10.
https://escholarship.umassmed.edu/witman/10