Isolation of Chlamydomonas flagella
Department of Cell and Developmental Biology
Cell and Developmental Biology | Cell Biology | Laboratory and Basic Science Research
A simple, scalable, and fast procedure for the isolation of Chlamydomonas flagella is described. Chlamydomonas can be synchronously deflagellated by treatment with chemicals, pH shock, or mechanical shear. The Basic Protocol describes the procedure for flagellar isolation using dibucaine to induce flagellar abscission; we also describe the pH shock method as an Alternate Protocol when flagellar regeneration is desirable. Sub-fractionation of the isolated flagella into axonemes and the membrane + matrix fraction is described in a Support Protocol.
DOI of Published Version
Craige, B., Brown, J. M. and Witman, G. B. 2013. Isolation of Chlamydomonas Flagella. Current Protocols in Cell Biology. 2013 Jun;Chapter 3:Unit 3.41.1-9. doi: 10.1002/0471143030.cb0341s59. Link to article on publisher's website
Current Protocols in Cell Biology
Craige B, Brown JM, Witman GB. (2013). Isolation of Chlamydomonas flagella. Witman Lab Publications. https://doi.org/10.1002/0471143030.cb0341s59. Retrieved from https://escholarship.umassmed.edu/witman/10