Department of Pediatrics; Department of Medicine; Center for Infectious Disease and Vaccine Research; Graduate School of Biomedical Sciences, Clinical and Population Health Research Program
Life Sciences | Medicine and Health Sciences | Women's Studies
Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity.
DOI of Published Version
Sci Rep. 2011;1:51. Epub 2011 Aug 1. Link to article on publisher's site
Friberg-Robertson, Heather L.; Bashyam, Hema Sundara; Toyosaki-Maeda, Tomoko; Potts, James A.; Greenough, Thomas C.; Kalayanarooj, Siripen; Gibbons, Robert V.; Nisalak, Ananda; Srikiatkhachorn, Anon; Green, Sharone; Stephens, Henry A. F.; Rothman, Alan L.; and Mathew, Anuja, "Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans" (2011). Women’s Health Research Faculty Publications. 517.