Department of Pediatrics; Department of Medicine; Center for Infectious Disease and Vaccine Research; Graduate School of Biomedical Sciences, Clinical and Population Health Research Program
Life Sciences | Medicine and Health Sciences | Women's Studies
Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity.
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DOI of Published Version
Sci Rep. 2011;1:51. Epub 2011 Aug 1. Link to article on publisher's site
Friberg-Robertson HL, Bashyam HS, Toyosaki-Maeda T, Potts JA, Greenough TC, Kalayanarooj S, Gibbons RV, Nisalak A, Srikiatkhachorn A, Green S, Stephens HA, Rothman AL, Mathew A. (2011). Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans. Women’s Health Research Faculty Publications. https://doi.org/10.1038/srep00051. Retrieved from https://escholarship.umassmed.edu/wfc_pp/517
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.