UMMS Affiliation
Department of Cell Biology; Department of Physiology; Program in Molecular Medicine
Publication Date
2006-08-02
Document Type
Article
Subjects
Adaptor Proteins, Signal Transducing; Animals; COS Cells; Cattle; Cells, Cultured; Cercopithecus aethiops; Down-Regulation; Focal Adhesions; Green Fluorescent Proteins; Humans; Membrane Proteins; Mice; Microfilament Proteins; Microtubule-Associated Proteins; Myocytes, Smooth Muscle; Nuclear Proteins; Protein Binding; Rats; Regulatory Sequences, Nucleic Acid; Transcription Factors
Disciplines
Cell Biology | Life Sciences | Medicine and Health Sciences
Abstract
Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that supervillin (SV)--a peripheral membrane protein that binds myosin II and F-actin in such cells--negatively regulates stress fibers, FAs, and cell-substrate adhesion. The major FA regulatory sequence within SV (SV342-571) binds to the LIM domains of two proteins in the zyxin family, thyroid receptor-interacting protein 6 (TRIP6) and lipoma-preferred partner (LPP), but not to zyxin itself. SV and TRIP6 colocalize within large FAs, where TRIP6 may help recruit SV. RNAi-mediated decreases in either protein increase cell adhesion to fibronectin. TRIP6 partially rescues SV effects on stress fibers and FAs, apparently by mislocating SV away from FAs. Thus, SV interactions with TRIP6 at FAs promote loss of FA structure and function. SV and TRIP6 binding partners suggest several specific mechanisms through which the SV-TRIP6 interaction may regulate FA maturation and/or disassembly.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's terms of use policy at: http://www.rupress.org/terms After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof.
DOI of Published Version
10.1083/jcb.200512051
Source
J Cell Biol. 2006 Jul 31;174(3):447-58. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of cell biology
Related Resources
PubMed ID
16880273
Repository Citation
Takizawa N, Smith TC, Nebl T, Crowley JL, Palmieri SJ, Lifshitz LM, Ehrhardt AG, Hoffman LM, Beckerle MC, Luna EJ. (2006). Supervillin modulation of focal adhesions involving TRIP6/ZRP-1. Women’s Health Research Faculty Publications. https://doi.org/10.1083/jcb.200512051. Retrieved from https://escholarship.umassmed.edu/wfc_pp/311
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.