Cloning, characterization, and chromosomal localization of human superillin (SVIL)
Department of Cell Biology
Amino Acid Sequence; Animals; Base Sequence; Cattle; Cell Line; Chromosome Mapping; Chromosomes, Human, Pair 10; Cloning, Molecular; Conserved Sequence; Gene Expression Regulation, Neoplastic; Humans; In Situ Hybridization, Fluorescence; Membrane Proteins; Microfilament Proteins; Molecular Sequence Data; Nuclear Localization Signals; RNA, Messenger; Sequence Analysis, DNA; Tumor Cells, Cultured
Cell Biology | Life Sciences | Medicine and Health Sciences
Supervillin is a 205-kDa F-actin binding protein originally isolated from bovine neutrophils. This protein is tightly associated with both actin filaments and plasma membranes, suggesting that it forms a high-affinity link between the actin cytoskeleton and the membrane. Human supervillin cDNAs cloned from normal human kidney and from the cervical carcinoma HeLa S3 predict a bipartite structure with three potential nuclear localization signals in the NH2-terminus and three potential actin-binding sequences in the COOH-terminus. In fact, throughout its length, the COOH-terminal half of supervillin is similar to segments 2-6 plus the COOH-terminal "headpiece" of villin, an actin-binding protein in intestinal microvilli. A comparison of the bovine and human sequences indicates that supervillin is highly conserved at the amino acid level, with 79.2% identity of the NH2-terminus and conservation of three of the four nuclear localization signals found in bovine supervillin. The COOH-terminus is even more conserved, with 95.1% amino acid identity overall and 100% conservation of the villin-like headpiece. Supervillin mRNAs are expressed in all human tissue tested, bu are most abundant in muscle, bone marrow, thyroid gland, and salivary gland; comparatively little message is found in brain. Human supervillin mRNA is approximately 7.5 kb; this message is especially abundant in HeLa S3 cervical carcinoma, SW480 adenocarcinoma, and A549 lung carcinoma cell lines. The human supervillin gene (SVIL) is localized to a single chromosomal locus at 10p11.2, a region that is deleted in some prostate tumors.
DOI of Published Version
Genomics. 1998 Sep 15;52(3):342-51.
Pope RK, Pestonjamasp KN, Smith KP, Wulfkuhle JD, Strassel CP, Lawrence JB, Luna EJ. (1998). Cloning, characterization, and chromosomal localization of human superillin (SVIL). Women’s Health Research Faculty Publications. https://doi.org/10.1006/geno.1998.5466. Retrieved from https://escholarship.umassmed.edu/wfc_pp/299