UMMS Affiliation
Department of Cell Biology
Publication Date
1981-02-01
Document Type
Article
Subjects
Actins; Cell Membrane; Dictyostelium; Membrane Lipids; Membrane Proteins; Polyethylene Glycols; Viscosity
Disciplines
Cell Biology | Life Sciences | Medicine and Health Sciences
Abstract
Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between these membranes and actin are examined by a novel application of falling ball viscometry. Treating the membranes as multivalent actin-binding particles analogous to divalent actin-gelation factors, we observe large increases in viscosity (actin cross-linking) when membranes of depleted actin and myosin are incubated with rabbit skeletal muscle F-actin. Pre-extraction of peripheral membrane proteins with chaotropes or the inclusion of Triton X-100 during the assay does not appreciably diminish this actin cross-linking activity. Lipid vesicles, heat-denatured membranes, proteolyzed membranes, or membranes containing endogenous actin show minimal actin cross-linking activity. Heat-denatured, but not proteolyzed, membranes regain activity when assayed in the presence of Triton X-100. Thus, integral membrane proteins appear to be responsible for some or all of the actin cross-linking activity of D. discoideum membranes. In the absence of MgATP, Triton X-100 extraction of isolated D. discoideum membranes results in a Triton-insoluble residue composed of actin, myosin, and associated membrane proteins. The inclusion of MgATP before and during Triton extraction greatly diminishes the amount of protein in the Triton-insoluble residue without appreciably altering its composition. Our results suggest the existence of a protein complex stabilized by actin and/or myosin (membrane cytoskeleton) associated with the D. discoideum plasma membrane.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's terms of use policy at: http://www.rupress.org/terms After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof.
DOI of Published Version
10.1083/jcb.88.2.396
Source
J Cell Biol. 1981 Feb;88(2):396-409. Link to article on publisher's website
Journal/Book/Conference Title
The Journal of cell biology
Related Resources
PubMed ID
6894148
Repository Citation
Luna, Elizabeth J.; Fowler, V. M.; Swanson, J.; Branton, D.; and Taylor, D. L., "A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity" (1981). Women’s Health Research Faculty Publications. 288.
https://escholarship.umassmed.edu/wfc_pp/288
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.
