UMMS Affiliation
Department of Cell Biology
Publication Date
1984-07-01
Document Type
Article
Subjects
Actins; Adenosine Triphosphate; Binding, Competitive; Cell Membrane; Cytoskeleton; Dictyostelium; Electrophoresis, Polyacrylamide Gel; Kinetics; Membrane Proteins; Microscopy, Electron; Myosin Subfragments; Myosins; Peptide Fragments
Disciplines
Cell Biology | Life Sciences | Medicine and Health Sciences
Abstract
The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads was found to be competitively inhibited by myosin subfragment-1. This inhibition is MgATP-sensitive, exhibits a Ki of approximately 5 X 10(-8) M, and is reciprocal, since membranes inhibit the binding of 125I-heavy meromyosin to F-actin on beads. These experiments demonstrate that membrane binding and S-1 binding to F-actin on beads are mutually exclusive and, therefore, that the membrane fragments bind predominantly to the sides, rather than to the ends, of the actin filaments. This conclusion is supported by electron micrographs that show many lateral associations between membrane fragments and bead-associated actin filaments. Such lateral associations could play an important role in the organization and lateral movement of membrane proteins by the cytomusculature.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's terms of use policy at: http://www.rupress.org/terms After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof.
DOI of Published Version
10.1083/jcb.99.1.71
Source
J Cell Biol. 1984 Jul;99(1 Pt 1):71-8. Link to article on publisher's website
Journal/Book/Conference Title
The Journal of cell biology
Related Resources
PubMed ID
6610683
Repository Citation
Goodloe-Holland, C. M. and Luna, Elizabeth J., "A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments" (1984). Women’s Health Research Faculty Publications. 286.
https://escholarship.umassmed.edu/wfc_pp/286
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.
