UMMS Affiliation
Department of Cell Biology
Publication Date
1986-06-01
Document Type
Article
Subjects
Actins; Binding Sites; Calcium-Binding Proteins; Cell Membrane; Cross-Linking Reagents; Cytoskeleton; Dictyostelium; Gelsolin; Intermediate Filaments; Kinetics; Microfilament Proteins; Phalloidine; Rhodamines; Staining and Labeling
Disciplines
Cell Biology | Life Sciences | Medicine and Health Sciences
Abstract
The binding of native, 125I-Bolton-Hunter-labeled actin to purified Dictyostelium discoideum plasma membranes was measured using a sedimentation assay. Binding was saturable only in the presence of the actin capping protein, gelsolin. In the presence of gelsolin, the amount of actin bound at saturation to three different membrane preparations was 80, 120, and 200 micrograms/mg of membrane protein. The respective concentrations of actin at half-saturation were 8, 12, and 18 micrograms/ml. The binding curves were sigmoidal, indicating positive cooperativity at low actin concentrations. This cooperativity appeared to be due to actin-actin associations during polymerization, since phalloidin converted the curve to a hyperbolic shape. In kinetic experiments, actin added as monomers bound to membranes at a rate of 0.6 microgram ml-1 min-1, while pre-polymerized actin bound at a rate of 3.0 micrograms ml-1 min-1. Even in the absence of phalloidin, actin bound to membranes at concentrations well below the normal critical concentration. This membrane-bound actin stained with rhodamine-phalloidin and was cross-linked by m-maleimidobenzoyl succinimide ester, a bifunctional cross-linker, into multimers with the same pattern observed for cross-linked F-actin. We conclude that D. discoideum plasma membranes bind actin specifically and saturably and that these membranes organize actin into filaments below the normal critical concentration for polymerization. This interaction probably occurs between multiple binding sites on the membrane and the side of the actin filament, and may be related to the clustering of membrane proteins.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's terms of use policy at: http://www.rupress.org/terms After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof.
DOI of Published Version
10.1083/jcb.102.6.2067
Source
J Cell Biol. 1986 Jun;102(6):2067-75. Link to article on publisher's website
Journal/Book/Conference Title
The Journal of cell biology
Related Resources
PubMed ID
2423531
Repository Citation
Schwartz, M. A. and Luna, Elizabeth J., "Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum" (1986). Women’s Health Research Faculty Publications. 281.
https://escholarship.umassmed.edu/wfc_pp/281
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.
