Title

Diacylglycerol-stimulated formation of actin nucleation sites at plasma membranes

UMMS Affiliation

Department of Cell Biology

Publication Date

1992-04-10

Document Type

Article

Subjects

Actins; Alkaloids; Animals; Calcium; Cell Membrane; Dictyostelium; Diglycerides; Kinetics; Macromolecular Substances; *Naphthalenes; Polycyclic Compounds; Protein Kinase C; Staurosporine; Tetradecanoylphorbol Acetate; Time Factors

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

Diacylglycerols, which are generated during phospholipase-catalyzed hydrolysis of phospholipids, stimulated actin polymerization in the presence of highly purified plasma membranes from the cellular slime mold Dictyostelium discoideum. The increased rate of actin polymerization apparently resulted from de novo formation of actin nucleation sites rather than uncapping of existing filament ends, because the membranes lacked detectable endogenous actin. The increased actin nucleation was mediated by a peripheral membrane component other than protein kinase C, the classical target of diacylglycerol action. These results indicate that diacylglycerols increase actin nucleation at plasma membranes and suggest a mechanism whereby signal transduction pathways may control cytoskeletal assembly.

DOI of Published Version

10.1126/science.1373523

Source

Science. 1992 Apr 10;256(5054):245-7.

Journal/Book/Conference Title

Science (New York, N.Y.)

Related Resources

Link to article in PubMed

PubMed ID

1373523

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