UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

2001-01-15

Document Type

Article

Subjects

Animals; B-Lymphocytes; Base Sequence; Cells, Cultured; DNA; DNA Primers; Gene Rearrangement, B-Lymphocyte, Heavy Chain; Hybridomas; *Immunoglobulin Switch Region; Mice; Mice, Knockout; Molecular Sequence Data; Recombination, Genetic; Sequence Deletion; Sequence Homology, Nucleic Acid; *Tandem Repeat Sequences

Disciplines

Life Sciences | Medicine and Health Sciences | Women's Studies

Abstract

Class switch DNA recombinations change the constant (C) region of the antibody heavy (H) chain expressed by a B cell and thereby change the antibody effector function. Unusual tandemly repeated sequence elements located upstream of H chain gene exons have long been thought to be important in the targeting and/or mechanism of the switch recombination process. We have deleted the entire switch tandem repeat element (S(mu)) from the murine (mu) H chain gene. We find that the S(mu) tandem repeats are not required for class switching in the mouse immunoglobulin H-chain locus, although the efficiency of switching is clearly reduced. Our data demonstrate that sequences outside of the S(mu) tandem repeats must be capable of directing the class switch mechanism. The maintenance of the highly repeated S(mu) element during evolution appears to reflect selection for a highly efficient switching process rather than selection for a required sequence element.

Keywords

gene rearrangement, B lymphocyte, heavy chain, class switching, immunoglobulin isotype

Rights and Permissions

Publisher PDF posted as allowed by the publisher's terms of use policy at: http://www.rupress.org/terms. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof.

DOI of Published Version

10.1084/jem.193.2.159

Source

J Exp Med. 2001 Jan 15;193(2):159-68.

Journal/Book/Conference Title

The Journal of experimental medicine

Related Resources

Link to article in PubMed

PubMed ID

11148220

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

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