UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyDocument Type
Journal ArticlePublication Date
2007-04-01Keywords
AnimalsAntigens, CD
B-Cell Activating Factor
B-Lymphocyte Subsets
B-Lymphocytes
Cell Proliferation
Female
Flow Cytometry
Immunoglobulin A
*Immunoglobulin Class Switching
Immunoglobulin G
Immunoglobulin M
Immunophenotyping
Interleukin-4
Interleukin-5
Lipopolysaccharides
Lymphocyte Activation
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Peyer's Patches
Species Specificity
Spleen
Transforming Growth Factor beta
Life Sciences
Medicine and Health Sciences
Women's Studies
Metadata
Show full item recordAbstract
Inbred mouse strains used for gene manipulation studies vary in many respects, including immune system function. These differences can interfere with data interpretation unless the mice are well backcrossed. Here, we show that antibody class switching to IgG3 in cultured splenic B cells from Swiss James Lambert (SJL) and 129/Sv mice is 2- to 6-fold less efficient compared with C57BL/6 (B6). Under optimal stimulation conditions, IgA switching is also 2- to 19-fold lower in SJL and 129/Sv B cells. Splenic B cells from SJL mice express higher levels of CD19 and CD21 compared with B6, and their CD21(high)CD23(low) B cells have little CD9 expression, suggesting atypical marginal zone (MZ) B cells. However, sort purification of splenic B cell subsets did not equalize in vitro class switching to IgG3 or IgA between SJL and B6. 129/Sv spleens have a 3-fold greater number of MZ B cells compared with B6, with similar CD9 expression. Poor IgG3 switching by 129/Sv B cells is specific to CD23(high) follicular B cells, whereas similar changes in IgA switching are seen in both CD21(high) and CD23(high) B cell subsets from 129/Sv. Therefore, the functions and phenotypes of mature B cells differ among three common strains of mice.Source
Int Immunol. 2007 Apr;19(4):545-56. Epub 2007 Feb 27. Link to article on publisher's siteDOI
10.1093/intimm/dxm020Permanent Link to this Item
http://hdl.handle.net/20.500.14038/50641PubMed ID
17329233Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1093/intimm/dxm020