Title

Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice

UMMS Affiliation

MassBiologics

Publication Date

2019-03-15

Document Type

Article

Disciplines

Bacteria | Bacterial Infections and Mycoses | Immunity | Immunology of Infectious Disease | Immunoprophylaxis and Therapy | Translational Medical Research

Abstract

We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of > 6 mug/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.

Keywords

DMAb, Lyme disease, OspA, human monoclonal antibody, UMCCTS funding

DOI of Published Version

10.1093/infdis/jiy627

Source

Wang Y, Esquivel R, Flingai S, Schiller ZA, Kern A, Agarwal S, Chu J, Patel A, Sullivan K, Wise MC, Broderick KE, Hu L, Weiner DB, Klempner MS. Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice. J Infect Dis. 2019 Mar 15;219(7):1146-1150. doi: 10.1093/infdis/jiy627. PMID: 30476132; PMCID: PMC6420172. Link to article on publisher's site

Journal/Book/Conference Title

The Journal of infectious diseases

Related Resources

Link to Article in PubMed

PubMed ID

30476132

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