UMMS Affiliation
Program in Molecular Medicine; Department of Microbiology and Physiological Systems
Publication Date
2014-07-01
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Cell Biology | Cells | Developmental Biology | Genetic Phenomena | Nucleic Acids, Nucleotides, and Nucleosides | Translational Medical Research
Abstract
Intestinal stem cells (ISCs) in the adult Drosophila midgut can respond to tissue damage and support repair. We used genetic manipulation to increase the number of ISC-like cells in the adult midgut and performed gene expression profiling to identify potential ISC regulators. A detailed analysis of one of these potential regulators, the zinc-finger protein Charlatan, was carried out. MARCM clonal analysis and RNAi in precursor cells showed that loss of Chn function caused severe ISC division defects, including loss of EdU incorporation, phosphorylated histone 3 staining and expression of the mitotic protein Cdc2. Loss of Charlatan also led to a much reduced histone acetylation staining in precursor cells. Both the histone acetylation and ISC division defects could be rescued by the simultaneous decrease of the Histone Deacetylase 2. The overexpression of Charlatan blocked differentiation reversibly, but loss of Charlatan did not lead to automatic differentiation. The results together suggest that Charlatan does not simply act as an anti-differentiation factor but instead functions to maintain a chromatin structure that is compatible with stem cell properties, including proliferation.
Keywords
Charlatan, Chromatin, Drosophila, Intestine, Stem cells, Zinc-finger protein, UMCCTS funding
Rights and Permissions
Copyright © 2014. Published by The Company of Biologists Ltd. Publisher's PDF posted after 12 months as allowed by publisher's open access policy at http://dev.biologists.org/content/rights-permissions.
DOI of Published Version
10.1242/dev.106237
Source
Development. 2014 Jul;141(13):2621-32. doi: 10.1242/dev.106237. Link to article on publisher's site
Journal/Book/Conference Title
Development (Cambridge, England)
Related Resources
PubMed ID
24961799
Repository Citation
Amcheslavsky, Alla; Nie, Yingchao; Li, Qi; He, Feng; Tsuda, Leo; Markstein, Michele; and Ip, Y. Tony, "Gene expression profiling identifies the zinc-finger protein Charlatan as a regulator of intestinal stem cells in Drosophila" (2014). UMass Center for Clinical and Translational Science Supported Publications. 188.
https://escholarship.umassmed.edu/umccts_pubs/188
Included in
Amino Acids, Peptides, and Proteins Commons, Cell Biology Commons, Cells Commons, Developmental Biology Commons, Genetic Phenomena Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons, Translational Medical Research Commons