Quantitative Human Immunodeficiency Virus (HIV)-1 Antibodies Correlate With Plasma HIV-1 RNA and Cell-associated DNA Levels in Children on Antiretroviral Therapy
Program in Molecular Medicine; Department of Quantitative Health Sciences
Immunology of Infectious Disease | Immunoprophylaxis and Therapy | Infectious Disease | Pediatrics | Therapeutics | Translational Medical Research | Virus Diseases | Viruses
Background: This study measured serial plasma human immunodeficiency virus (HIV)-1-specific antibody (Ab) levels in children who initiated antiretroviral therapy (ART) prior to 2 years of age, and evaluated their relationship to peripheral blood HIV-1 RNA and DNA levels.
Methods: We studied 46 HIV-1-infected children, stratified by age at ART initiation ( < 3 mo, early therapy [ET]; > 3 mo-2 years, late therapy [LT]) and by virologic response (R) or non-response (NR), before and up to 4 years following ART. We studied 20 HIV-1-uninfected children born to HIV-1-infected mothers (seroreverters [SR]) as controls. Plasma immunoglobulin G (IgG) Ab levels directed against HIV-1 envelope (gp160, gp41), gag (capsid, p24; matrix, p17), reverse transcriptase (p66/51), and integrase (p31) were serially measured using quantitative enzyme-linked immunosorbent assays. HIV-1 Ab rates of decline were estimated over the first 15 months of the study.
Results: The HIV-1 Ab rates of decline in the ET-R group were similar to those in the SR group for all Ab specificities, except for p17 (P = .01). Ab decline rates in the LT-R group and the NR group were significantly slower than in the SR group for all tested Ab specificities. After 1 year of age, Ab levels to p31 and p17 were significantly associated with HIV-1 RNA levels (P < .001); Ab levels to gp160 (P < .001) and gp41 (P < .001) were significantly associated with cell-associated HIV-1 DNA levels.
Conclusions: Quantitative HIV-1-specific Ab levels may be useful for screening children on ART for viral suppression or for residual, cell-associated HIV-1 DNA levels.
Clinical Trials Registration: NCT00000872.
pediatric early antiretroviral therapy, HIV-1 persistence, HIV-1 quantitative antibodies, UMCCTS funding
DOI of Published Version
Clin Infect Dis. 2018 Sep 5. pii: 5090954. doi: 10.1093/cid/ciy753. [Epub ahead of print] Link to article on publisher's site
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
McManus MM, Henderson JL, Gautam A, Brody RM, Weiss ER, Persaud D, Mick EO, Luzuriaga K, PACTG 356 Investigators. (2018). Quantitative Human Immunodeficiency Virus (HIV)-1 Antibodies Correlate With Plasma HIV-1 RNA and Cell-associated DNA Levels in Children on Antiretroviral Therapy. UMass Center for Clinical and Translational Science Supported Publications. https://doi.org/10.1093/cid/ciy753. Retrieved from https://escholarship.umassmed.edu/umccts_pubs/160