Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue
Department of Biochemistry and Molecular Pharmacology
Adenosine Triphosphate; Blotting, Western; Carbohydrate Sequence; Chemical Phenomena; Chemistry; Cloning, Molecular; Conserved Sequence; Diethyl Pyrocarbonate; Drug Resistance; Enterococcus; Histidine; Kanamycin Kinase; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligosaccharides; Phosphates; Phosphorylation; Phosphotransferases (Alcohol Group Acceptor); Protein Folding; Sequence Alignment; Staphylococcus
Biochemistry | Enzymes and Coenzymes | Medicinal-Pharmaceutical Chemistry | Therapeutics
BACKGROUND: The enzyme aminoglycoside 3'-phosphotransferase Type IIIa (APH(3')-IIIa), confers resistance to many aminoglycoside antibiotics by regiospecific phosphorylation of their hydroxyl groups. The chemical mechanism of phosphoryl transfer is unknown. Based on sequence homology, it has been suggested that a conserved His residue, His188, could be phosphorylated by ATP, and this phospho-His would transfer the phosphate to the incoming aminoglycoside. We have used chemical modification, site-directed mutagenesis and positional isotope exchange methods to probe the mechanism of phosphoryl transfer by APH(3')-IIIa.
RESULTS: Chemical modification by diethylpyrocarbonate implicated His in aminoglycoside phosphorylation by APH(3')-IIIa. We prepared His to Ala mutants of all four His residues in APH(3')-IIIa and found minimal effects of the mutations on the steady-state phosphorylation of several aminoglycosides. One of these mutants, His188Ala, was largely insoluble when compared to the wild-type enzyme. Positional isotope exchange experiments using gamma-[18O]-ATP did not support a double-displacement mechanism.
CONCLUSIONS: His residues are not required for aminoglycoside phosphorylation by APH(3')-IIIa. The conserved His 188 is thus not a phosphate accepting residue but does seem to be important for proper enzyme folding. Positional isotope exchange experiments are consistent with direct attack of the aminoglycoside hydroxyl group on the gamma-phosphate of ATP.
aminoglycoside, antibiotic resistance, mutagenesis, PIX
DOI of Published Version
Chem Biol. 1996 Sep;3(9):747-55. doi:10.1016/S1074-5521(96)90251-3
Chemistry and biology
Thompson, Paul R.; Hughes, D. W.; and Wright, G. D., "Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue" (1996). Thompson Lab Publications. 97.