Title
Mechanistic studies of agmatine deiminase from multiple bacterial species
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
2010-11-02
Document Type
Article
Subjects
Bacterial Proteins; Helicobacter pylori; Hydrogen-Ion Concentration; Hydrolases; Porphyromonas gingivalis; Protons; Streptococcus mutans
Disciplines
Biochemistry | Enzymes and Coenzymes | Medicinal-Pharmaceutical Chemistry | Therapeutics
Abstract
One subfamily of guanidino group-modifying enzymes (GMEs) consists of the agmatine deiminases (AgDs). These enzymes catalyze the conversion of agmatine (decarboxylated arginine) to N-carbamoyl putrescine and ammonia. In plants, viruses, and bacteria, these enzymes are thought to be involved in energy production, biosynthesis of polyamines, and biofilm formation. In particular, we are interested in the role that this enzyme plays in pathogenic bacteria. Previously, we reported the initial kinetic characterization of the agmatine deiminase from Helicobacter pylori and described the synthesis and characterization the two most potent AgD inactivators. Herein, we have expanded our initial efforts to characterize the catalytic mechanisms of AgD from H. pylori as well as Streptococcus mutans and Porphyromonas gingivalis. Through the use of pH rate profiles, pK(a) measurements of the active site cysteine, solvent isotope effects, and solvent viscosity effects, we have determined that the AgDs, like PADs 1 and 4, utilize a reverse protonation mechanism.
DOI of Published Version
10.1021/bi101405y
Source
Biochemistry. 2010 Nov 2;49(43):9413-23. doi: 10.1021/bi101405y. Link to article on publisher's site
Journal/Book/Conference Title
Biochemistry
Related Resources
Repository Citation
Jones JE, Dreyton CJ, Flick H, Causey CP, Thompson PR. (2010). Mechanistic studies of agmatine deiminase from multiple bacterial species. Thompson Lab Publications. https://doi.org/10.1021/bi101405y. Retrieved from https://escholarship.umassmed.edu/thompson/56
Comments
At the time of publication, Christina Dreyton and Paul Thompson were not yet affiliated with the University of Massachusetts Medical School.