Title
Development and use of clickable activity based protein profiling agents for protein arginine deiminase 4.
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
2011-05-20
Document Type
Article
Subjects
Amidines; Biotin; Enzyme Activation; Fluorescent Dyes; Humans; Hydrolases; Limit of Detection; Protein Array Analysis; Triazoles
Disciplines
Biochemistry | Enzymes and Coenzymes | Medicinal-Pharmaceutical Chemistry | Therapeutics
Abstract
The protein arginine deiminases (PADs), which catalyze the hydrolysis of peptidyl-arginine to form peptidyl-citrulline, are potential targets for the development of a rheumatoid arthritis (RA) therapeutic, as well as other human diseases including colitis and cancer. Additionally, these enzymes, and in particular PAD4, appear to play important roles in a variety of cell signaling pathways including apoptosis, differentiation, and transcriptional regulation. To better understand the factors that regulate in vivo PAD4 activity, we set out to design and synthesize a series of activity-based protein profiling (ABPP) reagents that target this enzyme. Herein we describe the design, synthesis, and evaluation of six ABPPs including (i) FITC-conjugated F-amidine (FFA1 and 2) and Cl-amidine (FCA1 and 2), and (ii) biotin-conjugated F-amidine (BFA) and Cl-amidine (BCA). We further demonstrate the utility of these probes for labeling PAD4 in cells, as well as for isolating PAD4 and PAD4 binding proteins. These probes will undoubtedly prove to be powerful tools that can be used to dissect the factors controlling the dynamics of PAD4 expression, activity, and function.
DOI of Published Version
10.1021/cb1003515
Source
ACS Chem Biol. 2011 May 20;6(5):466-76. doi: 10.1021/cb1003515. Epub 2011 Feb 7. Link to article on publisher's site
Journal/Book/Conference Title
ACS chemical biology
Related Resources
Repository Citation
Slack, Jessica L.; Causey, Corey P.; Luo, Yuan; and Thompson, Paul R., "Development and use of clickable activity based protein profiling agents for protein arginine deiminase 4." (2011). Thompson Lab Publications. 49.
https://escholarship.umassmed.edu/thompson/49
Comments
At the time of publication, Paul Thompson was not yet affiliated with UMass Medical School.