Title

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

5-20-2015

Document Type

Article

Disciplines

Biochemistry | Cancer Biology | Enzymes and Coenzymes | Laboratory and Basic Science Research | Medicinal-Pharmaceutical Chemistry | Therapeutics

Abstract

Here, we document the use of the soft agar colony formation assay to test the effects of a peptidylarginine deiminase (PADI) enzyme inhibitor, BB-Cl-amidine, on breast cancer tumorigenicity in vitro.

DOI of Published Version

10.3791/52727 (2015).

Source

Horibata, S., Vo, T. V., Subramanian, V., Thompson, P. R., Coonrod, S. A. Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells. J. Vis. Exp. (99), e52727, doi:10.3791/52727 (2015).

Journal/Book/Conference Title

Journal of Visualized Experiments : JoVE

Related Resources

Link to article in PubMed