Title
Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast
UMMS Affiliation
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Publication Date
6-1-2015
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology
Abstract
Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.
DOI of Published Version
10.1101/pdb.prot085191
Source
Cold Spring Harb Protoc. 2015 Jun 1;2015(6):593-8. doi: 10.1101/pdb.prot085191. Link to article on publisher's site
Journal/Book/Conference Title
Cold Spring Harbor protocols
Related Resources
PubMed ID
26034306
Repository Citation
Belton, Jon-Matthew and Dekker, Job, "Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast" (2015). Program in Systems Biology Publications and Presentations. 70.
https://escholarship.umassmed.edu/sysbio_pubs/70