Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology
Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.
DOI of Published Version
Cold Spring Harb Protoc. 2015 Jun 1;2015(6):593-8. doi: 10.1101/pdb.prot085191. Link to article on publisher's site
Cold Spring Harbor protocols
Belton J, Dekker J. (2015). Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast. Systems Biology Publications. https://doi.org/10.1101/pdb.prot085191. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/70