Title
Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast
UMMS Affiliation
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Publication Date
2015-06-01
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology
Abstract
Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.
DOI of Published Version
10.1101/pdb.prot085191
Source
Cold Spring Harb Protoc. 2015 Jun 1;2015(6):593-8. doi: 10.1101/pdb.prot085191. Link to article on publisher's site
Journal/Book/Conference Title
Cold Spring Harbor protocols
Related Resources
PubMed ID
26034306
Repository Citation
Belton J, Dekker J. (2015). Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.prot085191. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/70