Measuring Chromatin Structure in Budding Yeast
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology
Chromosome conformation capture (3C) has revolutionized the ways in which the conformation of chromatin and its relationship to other molecular functions can be studied. 3C-based techniques are used to determine the spatial arrangement of chromosomes in organisms ranging from bacteria to humans. In particular, they can be applied to the study of chromosome folding and organization in model organisms with small genomes and for which powerful genetic tools exist, such as budding yeast. Studies in yeast allow the mechanisms that establish or maintain chromatin structure to be analyzed at very high resolution with relatively low cost, and further our understanding of these fundamental processes in higher eukaryotes as well. Here we provide an overview of chromatin structure and introduce methods for performing 3C, with a focus on studies in budding yeast. Variations of the basic 3C approach (e.g., 3C-PCR, 5C, and Hi-C) can be used according to the scope and goals of a given experiment.
DOI of Published Version
Cold Spring Harb Protoc. 2015 Jul 1;2015(7):pdb.top077552. doi: 10.1101/pdb.top077552. Link to article on publisher's site
Cold Spring Harbor protocols
Belton, Jon-Matthew and Dekker, Job, "Measuring Chromatin Structure in Budding Yeast" (2015). Program in Systems Biology Publications and Presentations. 68.