UMMS Affiliation
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Publication Date
2015-07-21
Document Type
Article
Disciplines
Biochemistry | Genetics | Genomics | Molecular Biology | Structural Biology | Systems Biology
Abstract
We have examined the three-dimensional organization of the yeast genome during quiescence by a chromosome capture technique as a means of understanding how genome organization changes during development. For exponentially growing cells we observe high levels of inter-centromeric interaction but otherwise a predominance of intrachromosomal interactions over interchromosomal interactions, consistent with aggregation of centromeres at the spindle pole body and compartmentalization of individual chromosomes within the nucleoplasm. Three major changes occur in the organization of the quiescent cell genome. First, intrachromosomal associations increase at longer distances in quiescence as compared to growing cells. This suggests that chromosomes undergo condensation in quiescence, which we confirmed by microscopy by measurement of the intrachromosomal distances between two sites on one chromosome. This compaction in quiescence requires the condensin complex. Second, inter-centromeric interactions decrease, consistent with prior data indicating that centromeres disperse along an array of microtubules during quiescence. Third, inter-telomeric interactions significantly increase in quiescence, an observation also confirmed by direct measurement. Thus, survival during quiescence is associated with substantial topological reorganization of the genome.
DOI of Published Version
10.1093/nar/gkv723
Source
Nucleic Acids Res. 2015 Jul 21. pii: gkv723. doi: 10.1093/nar/gkv723. [Epub ahead of print] Link to article on publisher's site
Journal/Book/Conference Title
Nucleic acids research
Related Resources
PubMed ID
26202961
Repository Citation
Rutledge, Mark T.; Russo, Mariano; Belton, Jon-Matthew; Dekker, Job; and Broach, James R., "The yeast genome undergoes significant topological reorganization in quiescence" (2015). Program in Systems Biology Publications and Presentations. 65.
https://escholarship.umassmed.edu/sysbio_pubs/65
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Biochemistry Commons, Genetics Commons, Genomics Commons, Molecular Biology Commons, Structural Biology Commons, Systems Biology Commons
Comments
Copyright The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.