Title
RNA Interference in Caenorhabditis elegans
UMMS Affiliation
Program in Systems Biology; RNA Therapeutics Institute (RTI); Program in Molecular Medicine
Publication Date
2015-01-05
Document Type
Article
Disciplines
Genetics and Genomics | Laboratory and Basic Science Research | Molecular Biology | Systems Biology
Abstract
RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens. (c) 2015 by John Wiley and Sons, Inc.
DOI of Published Version
10.1002/0471142727.mb2603s109
Source
Curr Protoc Mol Biol. 2015 Jan 5;109:26.3.1-26.3.30. doi: 10.1002/0471142727.mb2603s109. Link to article on publisher's site
Journal/Book/Conference Title
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Related Resources
PubMed ID
25559107
Repository Citation
Conte, Darryl Jr.; MacNeil, Lesley T.; Walhout, Albertha J. M.; and Mello, Craig C., "RNA Interference in Caenorhabditis elegans" (2015). Program in Systems Biology Publications and Presentations. 62.
https://escholarship.umassmed.edu/sysbio_pubs/62