RNA Interference in Caenorhabditis elegans
Program in Systems Biology; RNA Therapeutics Institute (RTI); Program in Molecular Medicine
Genetics and Genomics | Laboratory and Basic Science Research | Molecular Biology | Systems Biology
RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens. (c) 2015 by John Wiley and Sons, Inc.
DOI of Published Version
Curr Protoc Mol Biol. 2015 Jan 5;109:26.3.1-26.3.30. doi: 10.1002/0471142727.mb2603s109. Link to article on publisher's site
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Conte, Darryl Jr.; MacNeil, Lesley T.; Walhout, Albertha J. M.; and Mello, Craig C., "RNA Interference in Caenorhabditis elegans" (2015). Program in Systems Biology Publications and Presentations. 62.