Widespread macromolecular interaction perturbations in human genetic disorders
Program in Systems Biology; Program in Molecular Medicine
Genetics | Molecular Biology | Molecular Genetics | Structural Biology | Systems Biology
How disease-associated mutations impair protein activities in the context of biological networks remains mostly undetermined. Although a few renowned alleles are well characterized, functional information is missing for over 100,000 disease-associated variants. Here we functionally profile several thousand missense mutations across a spectrum of Mendelian disorders using various interaction assays. The majority of disease-associated alleles exhibit wild-type chaperone binding profiles, suggesting they preserve protein folding or stability. While common variants from healthy individuals rarely affect interactions, two-thirds of disease-associated alleles perturb protein-protein interactions, with half corresponding to "edgetic" alleles affecting only a subset of interactions while leaving most other interactions unperturbed. With transcription factors, many alleles that leave protein-protein interactions intact affect DNA binding. Different mutations in the same gene leading to different interaction profiles often result in distinct disease phenotypes. Thus disease-associated alleles that perturb distinct protein activities rather than grossly affecting folding and stability are relatively widespread.
DOI of Published Version
Cell. 2015 Apr 23;161(3):647-60. doi: 10.1016/j.cell.2015.04.013. Link to article on publisher's site
Sahni, Nidhi; Yi, Song; Taipale, Mikko; Fuxman Bass, Juan; Coulombe-Huntington, Jasmin; Roth, Frederick P.; Xia, Yu; Walhout, Albertha J. M.; Lindquist, Susan; and Vidal, Marc, "Widespread macromolecular interaction perturbations in human genetic disorders" (2015). Program in Systems Biology Publications and Presentations. 59.