Title
The Hitchhiker's guide to Hi-C analysis: Practical guidelines
UMMS Affiliation
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Publication Date
2014-11-06
Document Type
Article
Disciplines
Bioinformatics | Computational Biology | Genetics and Genomics | Systems Biology
Abstract
Over the last decade, development and application of a set of molecular genomic approaches based on the chromosome conformation capture method (3C), combined with increasingly powerful imaging approaches, have enabled high resolution and genome-wide analysis of the spatial organization of chromosomes. The aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions.
Keywords
Bioinformatics, Chromatin structure, Chromosome conformation capture, Deep sequencing, Hi-C
DOI of Published Version
10.1016/j.ymeth.2014.10.031
Source
Methods. 2014 Nov 6. pii: S1046-2023(14)00358-2. doi: 10.1016/j.ymeth.2014.10.031. [Epub ahead of print]. Link to article on publisher's site
Journal/Book/Conference Title
Methods (San Diego, Calif.)
Related Resources
PubMed ID
25448293
Repository Citation
Lajoie BR, Dekker J, Kaplan N. (2014). The Hitchhiker's guide to Hi-C analysis: Practical guidelines. Program in Systems Biology Publications. https://doi.org/10.1016/j.ymeth.2014.10.031. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/55