Title

The Hitchhiker's guide to Hi-C analysis: Practical guidelines

UMMS Affiliation

Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

11-6-2014

Document Type

Article

Disciplines

Bioinformatics | Computational Biology | Genetics and Genomics | Systems Biology

Abstract

Over the last decade, development and application of a set of molecular genomic approaches based on the chromosome conformation capture method (3C), combined with increasingly powerful imaging approaches, have enabled high resolution and genome-wide analysis of the spatial organization of chromosomes. The aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions.

Keywords

Bioinformatics, Chromatin structure, Chromosome conformation capture, Deep sequencing, Hi-C

DOI of Published Version

10.1016/j.ymeth.2014.10.031

Source

Methods. 2014 Nov 6. pii: S1046-2023(14)00358-2. doi: 10.1016/j.ymeth.2014.10.031. [Epub ahead of print]. Link to article on publisher's site

Journal/Book/Conference Title

Methods (San Diego, Calif.)

Related Resources

Link to Article in PubMed

PubMed ID

25448293

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