UMMS Affiliation
Programs in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Publication Date
2013-11-15
Document Type
Article
Disciplines
Developmental Biology | Genetics and Genomics | Systems Biology
Abstract
V(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.
Keywords
B-cell development, Ag gene rearrangement, Gene expression
DOI of Published Version
10.1101/gad.227165.113
Source
Kumar S, Wuerffel R, Achour I, Lajoie B, Sen R, Dekker J, Feeney AJ, Kenter AL. Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny. Genes Dev. 2013 Nov 15;27(22):2439-44. doi: 10.1101/gad.227165.113. Link to article on publisher's site
Journal/Book/Conference Title
Genes and development
Related Resources
PubMed ID
24240234
Repository Citation
Kumar, Satyendra; Wuerffel, Robert; Achour, Ikbel; Lajoie, Bryan R.; Sen, Ranjan; Dekker, Job; Feeney, Ann J.; and Kenter, Amy L., "Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny" (2013). Program in Systems Biology Publications and Presentations. 37.
https://escholarship.umassmed.edu/sysbio_pubs/37
Comments
© 2013 Kumar et al.; Published by Cold Spring Harbor Laboratory Press.
This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.