Organization of the mitotic chromosome
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Computational Biology | Systems Biology
Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two distinct three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell type-specific organization described previously for nonsynchronous cells is restricted to interphase. In metaphase, we identified a homogenous folding state that is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we found that metaphase Hi-C data are inconsistent with classic hierarchical models and are instead best described by a linearly organized longitudinally compressed array of consecutive chromatin loops.
DOI of Published Version
Naumova N, Imakaev M, Fudenberg G, Zhan Y, Lajoie BR, Mirny LA, Dekker J. Organization of the mitotic chromosome. Science. 2013 Nov 22;342(6161):948-53. doi: 10.1126/science.1236083. Link to article on publisher's site
Science (New York, N.Y.)
Naumova, Natalia; Imakaev, Maxim; Fudenberg, Geoffrey; Zhan, Ye; Lajoie, Bryan R.; Mirny, Leonid A.; and Dekker, Job, "Organization of the mitotic chromosome" (2013). Program in Systems Biology Publications and Presentations. 36.