Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Progeria; Nuclear Lamina; Heterochromatin; Nuclear Proteins
Congenital, Hereditary, and Neonatal Diseases and Abnormalities | Genetics and Genomics | Systems Biology
Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disease that is frequently caused by a de novo point mutation at position 1824 in LMNA. This mutation activates a cryptic splice donor site in exon 11, and leads to an in-frame deletion within the prelamin A mRNA and the production of a dominant negative lamin A protein, known as progerin. Here we show that primary HGPS skin fibroblasts experience genome-wide correlated alterations in patterns of H3K27me3 deposition, DNA-lamin A/C associations, and, at late passages, genome-wide loss of spatial compartmentalization of active and inactive chromatin domains. We further demonstrate that the H3K27me3 changes associate with gene expression alterations in HGPS cells. Our results support a model that the accumulation of progerin in the nuclear lamina leads to altered H3K27me3 marks in heterochromatin, possibly through the down-regulation of EZH2, and disrupts heterochromatin-lamina interactions. These changes may result in transcriptional misregulation and eventually trigger the global loss of spatial chromatin compartmentalization in late passage HGPS fibroblasts.
DOI of Published Version
Genome Res. 2013 Feb;23(2):260-9. doi: 10.1101/gr.138032.112. Epub 2012 Nov 14. Link to article on publisher's site
McCord, Rachel Patton; Nazario-Toole, Ashley; Zhang, Haoyue; Chines, Peter; Zhan, Ye; Erdos, Michael; Collins, Francis; Dekker, Job; and Cao, Kan, "Correlated alterations in genome organization, histone methylation, and DNA-lamin A/C interactions in Hutchinson-Gilford progeria syndrome" (2013). Program in Systems Biology Publications and Presentations. 16.