Title
A Delicate Balance between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development
UMMS Affiliation
Program in Systems Biology; Program in Molecular Medicine; Graduate School of Biomedical Sciences
Publication Date
2019-09-11
Document Type
Article
Disciplines
Bacteria | Biochemical Phenomena, Metabolism, and Nutrition | Cell Biology | Cellular and Molecular Physiology | Developmental Biology | Genetic Phenomena | Genetics and Genomics | Systems Biology
Abstract
Iron is an essential micronutrient for all forms of life; low levels of iron cause human disease, while too much iron is toxic. Low iron levels induce reactive oxygen species (ROS) by disruption of the heme and iron-sulfur cluster-dependent electron transport chain (ETC). To identify bacterial metabolites that affect development, we screened the Keio Escherichia coli collection and uncovered 244 gene deletion mutants that slow Caenorhabditis elegans development. Several of these genes encode members of the ETC cytochrome bo oxidase complex, as well as iron importers. Surprisingly, either iron or anti-oxidant supplementation reversed the developmental delay. This suggests that low bacterial iron results in high bacterial ROS and vice versa, which causes oxidative stress in C. elegans that subsequently impairs mitochondrial function and delays development. Our data indicate that the bacterial diets of C. elegans provide precisely tailored amounts of iron to support proper development.
Keywords
C. elegans, E. coli, diet, electron transport chain, flux balance analysis, iron, metabolic network modeling, metabolism, reactive oxygen species
DOI of Published Version
10.1016/j.chom.2019.07.010
Source
Cell Host Microbe. 2019 Sep 11;26(3):400-411.e3. doi: 10.1016/j.chom.2019.07.010. Epub 2019 Aug 20. Link to article on publisher's site
Journal/Book/Conference Title
Cell host and microbe
Related Resources
PubMed ID
31444089
Repository Citation
Zhang J, Li X, Olmedo M, Holdorf AD, Shang Y, Artal-Sanz M, Yilmaz LS, Walhout AJ. (2019). A Delicate Balance between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development. Systems Biology Publications. https://doi.org/10.1016/j.chom.2019.07.010. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/156