3C-Based Chromatin Interaction Analyses
Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology
Computational Biology | Genetic Phenomena | Genomics | Investigative Techniques | Laboratory and Basic Science Research | Molecular Biology | Structural Biology | Systems Biology
This introduction presents a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function-chromosome conformation capture (3C). This approach allows us to determine the spatial proximity of distant functional genomic sites (by looping). 3C-based techniques to interrogate chromosome folding and long-range interactions between genomic sequences in vivo are detailed.
DOI of Published Version
Cold Spring Harb Protoc. 2018 Sep 4;2018(9):pdb.top097832. doi: 10.1101/pdb.top097832. Link to article on publisher's site
Cold Spring Harbor protocols
Kim, Tae Hoon and Dekker, Job, "3C-Based Chromatin Interaction Analyses" (2018). Program in Systems Biology Publications and Presentations. 149.