Title

3C-Based Chromatin Interaction Analyses

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology

Publication Date

2018-09-04

Document Type

Article

Disciplines

Computational Biology | Genetic Phenomena | Genomics | Investigative Techniques | Laboratory and Basic Science Research | Molecular Biology | Structural Biology | Systems Biology

Abstract

This introduction presents a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function-chromosome conformation capture (3C). This approach allows us to determine the spatial proximity of distant functional genomic sites (by looping). 3C-based techniques to interrogate chromosome folding and long-range interactions between genomic sequences in vivo are detailed.

DOI of Published Version

10.1101/pdb.top097832

Source

Cold Spring Harb Protoc. 2018 Sep 4;2018(9):pdb.top097832. doi: 10.1101/pdb.top097832. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

30181229

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