Document Type
Journal ArticlePublication Date
2018-07-02Keywords
Biochemistry, Biophysics, and Structural BiologyGenetics and Genomics
Laboratory and Basic Science Research
Systems Biology
Metadata
Show full item recordAbstract
Generating DNA-binding domain (DB)-bait strains for Gateway-compatible yeast two-hybrid (Y2H) screens involves three steps. The first is to generate an Entry clone containing a DNA fragment encoding the protein of interest (e.g., an open reading frame, ORF). The second is to transfer this DNA fragment from the Entry clone to the Y2H Destination vector, pDEST32. The final step is to transform this construct into the Y2H yeast strain, MaV103. This protocol takes 24-37 d plus sequence confirmation, if necessary, to complete.Source
Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.prot094979. doi: 10.1101/pdb.prot094979. Link to article on publisher's site
DOI
10.1101/pdb.prot094979Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49871PubMed ID
29967272Related Resources
ae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot094979