Title

High-Efficiency Yeast Transformation

UMMS Affiliation

Program in Systems Biology; Program in Molecular Medicine

Publication Date

2018-07-02

Document Type

Article

Disciplines

Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology

Abstract

High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.

DOI of Published Version

10.1101/pdb.prot094995

Source

Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.prot094995. doi: 10.1101/pdb.prot094995.Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

29967274

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