Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
Program in Systems Biology; Program in Molecular Medicine
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology
In the first section of this introduction, we provide background information for yeast two-hybrid (Y2H) assays that provide a genetic method for the identification and analysis of binary protein-protein interactions and that are complementary to biochemical methods such as immunoprecipitation. In the second section, we discuss yeast one-hybrid (Y1H) assays that provide a "gene-centered" (DNA-to-protein) genetic method to identify and study protein-DNA interactions between cis-regulatory elements and transcription factors (TFs). This method is complementary to "TF-centered" (protein-to-DNA) biochemical methods such as chromatin immunoprecipitation.
DOI of Published Version
Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.top094953. doi: 10.1101/pdb.top094953. Link to article on publisher's site
Cold Spring Harbor protocols
Reece-Hoyes JS, Walhout AJ. (2018). Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.top094953. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/140