Title

Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays

UMMS Affiliation

Program in Systems Biology; Program in Molecular Medicine

Publication Date

2018-07-02

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology

Abstract

In the first section of this introduction, we provide background information for yeast two-hybrid (Y2H) assays that provide a genetic method for the identification and analysis of binary protein-protein interactions and that are complementary to biochemical methods such as immunoprecipitation. In the second section, we discuss yeast one-hybrid (Y1H) assays that provide a "gene-centered" (DNA-to-protein) genetic method to identify and study protein-DNA interactions between cis-regulatory elements and transcription factors (TFs). This method is complementary to "TF-centered" (protein-to-DNA) biochemical methods such as chromatin immunoprecipitation.

DOI of Published Version

10.1101/pdb.top094953

Source

Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.top094953. doi: 10.1101/pdb.top094953. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

29967278

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