Generation of ChIP-Loop Libraries
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology
The ChIP-loop method combines the standard 3C protocol with a routine ChIP protocol. This method allows the selective identification of long-range chromatin interactions between loci that are bound to specific proteins of interest. ChIP-loop experiments need to be designed and planned as described for the standard 3C protocol. First, identify a restriction enzyme that digests chromatin so that the elements of interest are located on separate restriction fragments. Second, determine whether an antibody is available that successfully immunoprecipitates the protein of interest in a typical ChIP experiment.
DOI of Published Version
Cold Spring Harb Protoc. 2018 Aug 1;2018(8):pdb.prot097857. doi: 10.1101/pdb.prot097857. Link to article on publisher's site
Cold Spring Harbor protocols
Kim TH, Dekker J. (2018). Generation of ChIP-Loop Libraries. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.prot097857. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/137