UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Systems Biology
Document Type
Journal ArticlePublication Date
2018-08-01Keywords
Biochemistry, Biophysics, and Structural BiologyGenetics and Genomics
Laboratory and Basic Science Research
Systems Biology
Metadata
Show full item recordAbstract
The ChIP-loop method combines the standard 3C protocol with a routine ChIP protocol. This method allows the selective identification of long-range chromatin interactions between loci that are bound to specific proteins of interest. ChIP-loop experiments need to be designed and planned as described for the standard 3C protocol. First, identify a restriction enzyme that digests chromatin so that the elements of interest are located on separate restriction fragments. Second, determine whether an antibody is available that successfully immunoprecipitates the protein of interest in a typical ChIP experiment.Source
Cold Spring Harb Protoc. 2018 Aug 1;2018(8):pdb.prot097857. doi: 10.1101/pdb.prot097857. Link to article on publisher's site
DOI
10.1101/pdb.prot097857Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49865PubMed ID
30068591Related Resources
ae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot097857