Title

Generation of ChIP-Loop Libraries

UMMS Affiliation

Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

2018-08-01

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Laboratory and Basic Science Research | Systems Biology

Abstract

The ChIP-loop method combines the standard 3C protocol with a routine ChIP protocol. This method allows the selective identification of long-range chromatin interactions between loci that are bound to specific proteins of interest. ChIP-loop experiments need to be designed and planned as described for the standard 3C protocol. First, identify a restriction enzyme that digests chromatin so that the elements of interest are located on separate restriction fragments. Second, determine whether an antibody is available that successfully immunoprecipitates the protein of interest in a typical ChIP experiment.

DOI of Published Version

10.1101/pdb.prot097857

Source

Cold Spring Harb Protoc. 2018 Aug 1;2018(8):pdb.prot097857. doi: 10.1101/pdb.prot097857. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

30068591

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