Preparation of Cross-Linked Chromatin for ChIP
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Laboratory and Basic Science Research | Molecular Biology | Systems Biology
After living cells have been treated with formaldehyde to lock proteins to their DNA substrates, the DNA-protein complexes must be extracted and fragmented as described here. For ChIP-chip, it is not essential that a majority of chromatin fragments are approximately 500 bp; an average fragment size ranging 1-2 kb is sufficient. However, for ChIP-seq, the average chromatin fragment should bebp.
DOI of Published Version
Cold Spring Harb Protoc. 2018 Apr 2;2018(4):pdb.prot082602. doi: 10.1101/pdb.prot082602. Link to article on publisher's site
Cold Spring Harbor protocols
Kim TH, Dekker J. (2018). Preparation of Cross-Linked Chromatin for ChIP. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.prot082602. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/133