Title

Preparation of Cross-Linked Chromatin for ChIP

UMMS Affiliation

Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

4-2-2018

Document Type

Article

Disciplines

Laboratory and Basic Science Research | Molecular Biology | Systems Biology

Abstract

After living cells have been treated with formaldehyde to lock proteins to their DNA substrates, the DNA-protein complexes must be extracted and fragmented as described here. For ChIP-chip, it is not essential that a majority of chromatin fragments are approximately 500 bp; an average fragment size ranging 1-2 kb is sufficient. However, for ChIP-seq, the average chromatin fragment should bebp.

DOI of Published Version

10.1101/pdb.prot082602

Source

Cold Spring Harb Protoc. 2018 Apr 2;2018(4):pdb.prot082602. doi: 10.1101/pdb.prot082602. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

29610358

Share

COinS