Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Laboratory and Basic Science Research | Molecular Biology | Systems Biology
ChIP is a powerful method for analyzing protein-DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are approximately 10-fold higher than for a standard ChIP assay.
DOI of Published Version
Cold Spring Harb Protoc. 2018 Apr 2;2018(4):pdb.prot082610. doi: 10.1101/pdb.prot082610. Link to article on publisher's site
Cold Spring Harbor protocols
Kim TH, Dekker J. (2018). ChIP. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.prot082610. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/132