Title

ChIP

UMMS Affiliation

Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

4-2-2018

Document Type

Article

Disciplines

Laboratory and Basic Science Research | Molecular Biology | Systems Biology

Abstract

ChIP is a powerful method for analyzing protein-DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are approximately 10-fold higher than for a standard ChIP assay.

DOI of Published Version

10.1101/pdb.prot082610

Source

Cold Spring Harb Protoc. 2018 Apr 2;2018(4):pdb.prot082610. doi: 10.1101/pdb.prot082610. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

29610359

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