Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Laboratory and Basic Science Research | Molecular Biology | Systems Biology
ChIP-chip can be used to analyze protein-DNA interactions in a region-wide and genome-wide manner. DNA microarrays contain PCR products or oligonucleotide probes that are designed to represent genomic sequences. Identification of genomic sites that interact with a specific protein is based on competitive hybridization of the ChIP-enriched DNA and the input DNA to DNA microarrays. The ChIP-chip protocol can be divided into two main sections: Amplification of ChIP DNA and hybridization of ChIP DNA to arrays. A large amount of DNA is required to hybridize to DNA arrays, and hybridization to a set of multiple commercial arrays that represent the entire human genome requires two rounds of PCR amplifications. The relative hybridization intensity of ChIP DNA and that of the input DNA is used to determine whether the probe sequence is a potential site of protein-DNA interaction. Resolution of actual genomic sites bound by the protein is dependent on the size of the chromatin and on the genomic distance between the probes on the array. As with expression profiling using gene chips, ChIP-chip experiments require multiple replicates for reliable statistical measure of protein-DNA interactions.
DOI of Published Version
Cold Spring Harb Protoc. 2018 May 1;2018(5):pdb.prot082636. doi: 10.1101/pdb.prot082636. Link to article on publisher's site
Cold Spring Harbor protocols
Kim TH, Dekker J. (2018). ChIP-chip. Systems Biology Publications. https://doi.org/10.1101/pdb.prot082636. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/129