Chromatin Immunoprecipitation (ChIP) Analysis of Protein-DNA Interactions
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Laboratory and Basic Science Research | Molecular Biology | Systems Biology
Here we describe chromatin immunoprecipitation (ChIP), a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function. This approach allows us to determine the distribution of histone modifications (e.g., acetylation, methylation), the deposition of histone variants (H2AZ, H3.3, etc.), and the location of sequence-specific and general transcription factors. We introduce well-established ChIP-based methods that allow analysis of protein-DNA interactions in living cells.
DOI of Published Version
Cold Spring Harb Protoc. 2018 May 1;2018(5):pdb.top082586. doi: 10.1101/pdb.top082586. Link to article on publisher's site
Cold Spring Harbor protocols
Kim TH, Dekker J. (2018). Chromatin Immunoprecipitation (ChIP) Analysis of Protein-DNA Interactions. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.top082586. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/127