Title

Chromatin Immunoprecipitation (ChIP) Analysis of Protein-DNA Interactions

UMMS Affiliation

Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

5-1-2018

Document Type

Article

Disciplines

Laboratory and Basic Science Research | Molecular Biology | Systems Biology

Abstract

Here we describe chromatin immunoprecipitation (ChIP), a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function. This approach allows us to determine the distribution of histone modifications (e.g., acetylation, methylation), the deposition of histone variants (H2AZ, H3.3, etc.), and the location of sequence-specific and general transcription factors. We introduce well-established ChIP-based methods that allow analysis of protein-DNA interactions in living cells.

DOI of Published Version

10.1101/pdb.top082586

Source

Cold Spring Harb Protoc. 2018 May 1;2018(5):pdb.top082586. doi: 10.1101/pdb.top082586. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

29717056

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