Title
Chromatin Immunoprecipitation (ChIP) Analysis of Protein-DNA Interactions
UMMS Affiliation
Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Publication Date
5-1-2018
Document Type
Article
Disciplines
Laboratory and Basic Science Research | Molecular Biology | Systems Biology
Abstract
Here we describe chromatin immunoprecipitation (ChIP), a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function. This approach allows us to determine the distribution of histone modifications (e.g., acetylation, methylation), the deposition of histone variants (H2AZ, H3.3, etc.), and the location of sequence-specific and general transcription factors. We introduce well-established ChIP-based methods that allow analysis of protein-DNA interactions in living cells.
DOI of Published Version
10.1101/pdb.top082586
Source
Cold Spring Harb Protoc. 2018 May 1;2018(5):pdb.top082586. doi: 10.1101/pdb.top082586. Link to article on publisher's site
Journal/Book/Conference Title
Cold Spring Harbor protocols
Related Resources
PubMed ID
29717056
Repository Citation
Kim, Tae Hoon and Dekker, Job, "Chromatin Immunoprecipitation (ChIP) Analysis of Protein-DNA Interactions" (2018). Program in Systems Biology Publications and Presentations. 127.
https://escholarship.umassmed.edu/sysbio_pubs/127