Title

C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2

UMMS Affiliation

RNA Therapeutics Institute; Proteomics and Mass Spectrometry Facility; Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology; Department of Molecular, Cell and Cancer Biology; Program in Molecular Medicine

Publication Date

5-7-2018

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Biochemistry | Genetics and Genomics | Molecular Biology | Structural Biology | Systems Biology

Abstract

Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.

DOI of Published Version

10.1038/s41592-018-0006-2

Source

Nat Methods. 2018 May 7. doi: 10.1038/s41592-018-0006-2. Link to article on publisher's site

Journal/Book/Conference Title

Nature methods

Comments

The preprint version of this article as posted in bioRxiv is available.

Related Resources

Link to Article in PubMed

PubMed ID

29735996

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