C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
RNA Therapeutics Institute; Proteomics and Mass Spectrometry Facility; Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology; Department of Molecular, Cell and Cancer Biology; Program in Molecular Medicine
Amino Acids, Peptides, and Proteins | Biochemistry | Genetics and Genomics | Molecular Biology | Structural Biology | Systems Biology
Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.
DOI of Published Version
Nat Methods. 2018 May 7. doi: 10.1038/s41592-018-0006-2. Link to article on publisher's site
Gao, Xin D.; Tu, Li-Chun; Mir, Aamir; Tomas Rodriguez; Ding, Yue-He; Leszyk, John D.; Dekker, Job; Shaffer, Scott A.; Zhu, Lihua Julie; Wolfe, Scot A.; and Sontheimer, Erik J., "C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2" (2018). Program in Systems Biology Publications and Presentations. 126.