Title
C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
UMMS Affiliation
RNA Therapeutics Institute; Proteomics and Mass Spectrometry Facility; Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology; Department of Molecular, Cell and Cancer Biology; Program in Molecular Medicine
Publication Date
2018-05-07
Document Type
Article
Disciplines
Amino Acids, Peptides, and Proteins | Biochemistry | Genetics and Genomics | Molecular Biology | Structural Biology | Systems Biology
Abstract
Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.
DOI of Published Version
10.1038/s41592-018-0006-2
Source
Nat Methods. 2018 May 7. doi: 10.1038/s41592-018-0006-2. Link to article on publisher's site
Journal/Book/Conference Title
Nature methods
Related Resources
PubMed ID
29735996
Repository Citation
Gao XD, Tu L, Mir A, T, Ding Y, Leszyk JD, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ. (2018). C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2. Program in Systems Biology Publications. https://doi.org/10.1038/s41592-018-0006-2. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/126
Comments
The preprint version of this article as posted in bioRxiv is available.