C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
RNA Therapeutics Institute; Proteomics and Mass Spectrometry Facility; Department of Biochemistry and Molecular Pharmacology; Program in Systems Biology; Department of Molecular, Cell and Cancer Biology; Program in Molecular Medicine
Amino Acids, Peptides, and Proteins | Biochemistry | Genetics and Genomics | Molecular Biology | Structural Biology | Systems Biology
Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.
DOI of Published Version
Nat Methods. 2018 May 7. doi: 10.1038/s41592-018-0006-2. Link to article on publisher's site
Gao XD, Tu L, Mir A, T, Ding Y, Leszyk JD, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ. (2018). C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2. Program in Systems Biology Publications. https://doi.org/10.1038/s41592-018-0006-2. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/126