Program in Systems Biology; Program in Molecular Medicine; UMass Metabolic Network
Cellular and Molecular Physiology | Developmental Biology | Systems Biology
Vitamin B12 functions as a cofactor for methionine synthase to produce the anabolic methyl donor S-adenosylmethionine (SAM) and for methylmalonyl-CoA mutase to catabolize the short-chain fatty acid propionate. In the nematode Caenorhabditis elegans, maternally supplied vitamin B12 is required for the development of offspring. However, the mechanism for exporting vitamin B12 from the mother to the offspring is not yet known. Here, we use RNAi of more than 200 transporters with a vitamin B12-sensor transgene to identify the ABC transporter MRP-5 as a candidate vitamin B12 exporter. We show that the injection of vitamin B12 into the gonad of mrp-5 deficient mothers rescues embryonic lethality in the offspring. Altogether, our findings identify a maternal mechanism for the transit of an essential vitamin to support the development of the next generation.
ABC transporter, Caenorhabditis elegans, RNAi screen, mrp-5, vitamin B12
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Copyright 2018 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI of Published Version
Cell Rep. 2018 Mar 20;22(12):3126-3133. doi: 10.1016/j.celrep.2018.02.100. Link to article on publisher's site
Na H, Ponomarova O, Giese GE, Walhout AJ. (2018). C. elegans MRP-5 Exports Vitamin B12 from Mother to Offspring to Support Embryonic Development. Program in Systems Biology Publications and Presentations. https://doi.org/10.1016/j.celrep.2018.02.100. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/124
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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.