Title
Yeast one-hybrid assays: A historical and technical perspective
UMMS Affiliation
Program in Systems Biology
Publication Date
2012-08-03
Document Type
Article
Subjects
Two-Hybrid System Techniques
Disciplines
Genetics and Genomics | Systems Biology
Abstract
Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an important technique for detecting physical interactions between sequence-specific regulatory transcription factor proteins (TFs) and their DNA target sites. Multiple versions of the Y1H methodology have been developed, each with technical differences and unique advantages. We will discuss several of these technical variations in detail, and also provide some ideas for how Y1H assays can be further improved.
DOI of Published Version
10.1016/j.ymeth.2012.07.027
Source
Methods. 2012 Aug 3. [Epub ahead of print]. doi:10.1016/j.ymeth.2012.07.027
Journal/Book/Conference Title
Methods (San Diego, Calif.)
Related Resources
PubMed ID
22884952
Repository Citation
Reece-Hoyes, John S. and Walhout, Albertha J. M., "Yeast one-hybrid assays: A historical and technical perspective" (2012). Program in Systems Biology Publications and Presentations. 12.
https://escholarship.umassmed.edu/sysbio_pubs/12