Title

Yeast one-hybrid assays: A historical and technical perspective

UMMS Affiliation

Program in Systems Biology

Publication Date

2012-08-03

Document Type

Article

Subjects

Two-Hybrid System Techniques

Disciplines

Genetics and Genomics | Systems Biology

Abstract

Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an important technique for detecting physical interactions between sequence-specific regulatory transcription factor proteins (TFs) and their DNA target sites. Multiple versions of the Y1H methodology have been developed, each with technical differences and unique advantages. We will discuss several of these technical variations in detail, and also provide some ideas for how Y1H assays can be further improved.

DOI of Published Version

10.1016/j.ymeth.2012.07.027

Source

Methods. 2012 Aug 3. [Epub ahead of print]. doi:10.1016/j.ymeth.2012.07.027

Journal/Book/Conference Title

Methods (San Diego, Calif.)

Related Resources

Link to article in PubMed

PubMed ID

22884952

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