Title

Gateway Recombinational Cloning

UMMS Affiliation

Program in Systems Biology; Program in Molecular Medicine

Publication Date

2018-01-02

Document Type

Article

Disciplines

Genetic Phenomena | Genetics and Genomics | Genetic Structures | Investigative Techniques | Laboratory and Basic Science Research | Molecular Biology | Systems Biology

Abstract

The Gateway recombinatorial cloning system was developed for cloning multiple DNA fragments in parallel (e.g., in 96-well formats) in a standardized manner using the same enzymes. Gateway cloning is based on the highly specific integration and excision reactions of bacteriophage lambda into and out of the Escherichia coli genome. Because the sites of recombination ("att" sites) are much longer (25-242 bp) than restriction sites, they are extremely unlikely to occur by chance in DNA fragments. Therefore, the same recombination enzyme can be used to robustly clone many different fragments of variable size in parallel reactions.

DOI of Published Version

10.1101/pdb.top094912

Source

Cold Spring Harb Protoc. 2018 Jan 2;2018(1):pdb.top094912. doi: 10.1101/pdb.top094912. Link to article on publisher's site

Journal/Book/Conference Title

Cold Spring Harbor protocols

Related Resources

Link to Article in PubMed

PubMed ID

29295908

Share

COinS