Title

A pathway for mitotic chromosome formation

UMMS Affiliation

Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

2-9-2018

Document Type

Article

Disciplines

Biochemistry | Computational Biology | Molecular Biology | Structural Biology | Systems Biology

Abstract

Mitotic chromosomes fold as compact arrays of chromatin loops. To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed. During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops. The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold. The size of helical turns progressively increases to ~12 megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding.

DOI of Published Version

10.1126/science.aao6135

Source

Science. 2018 Feb 9;359(6376). pii: eaao6135. doi: 10.1126/science.aao6135. Epub 2018 Jan 18. Link to article on publisher's site

Journal/Book/Conference Title

Science (New York, N.Y.)

Related Resources

Link to Article in PubMed

PubMed ID

29348367

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