Gene-Centered Yeast One-Hybrid Assays
Program in Systems Biology; Program in Molecular Medicine; UMass Metabolic Network
Genetics and Genomics | Laboratory and Basic Science Research | Molecular Biology | Systems Biology
An important question when studying gene regulation is which transcription factors (TFs) interact with which cis-regulatory elements, such as promoters and enhancers. Addressing this issue in complex multicellular organisms is challenging as several hundreds of TFs and thousands of regulatory elements must be considered in the context of different tissues and physiological conditions. Yeast one-hybrid (Y1H) assays provide a powerful "gene-centered" method to identify the TFs that can bind a DNA sequence of interest. In this introduction, we describe the basic principles of the Y1H assay and its advantages and disadvantages and briefly discuss how it is complementary to "TF-centered" methods that identify protein-DNA interactions for a known protein of interest.
DOI of Published Version
Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.top077669. doi: 10.1101/pdb.top077669. Link to article on publisher's site
Cold Spring Harbor protocols
Fuxman Bass J, Reece-Hoyes JS, Walhout AJ. (2016). Gene-Centered Yeast One-Hybrid Assays. Program in Systems Biology Publications. https://doi.org/10.1101/pdb.top077669. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/103