Enhanced Y1H assays for Arabidopsis
Program in Gene Function and Expression; Program in Molecular Medicine
Arabidopsis; Gene Expression Regulation, Plant; Plant Roots; Promoter Regions, Genetic; Reproducibility of Results; Transcription Factors; *Two-Hybrid System Techniques
Molecular Biology | Plant Sciences | Systems Biology
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
DOI of Published Version
Nat Methods. 2011 Oct 30;8(12):1053-5. doi: 10.1038/nmeth.1750. Link to article on publisher's site
Gaudinier A, Zhang L, Reece-Hoyes JS, Taylor-Teeples M, Pu L, Liu Z, Breton G, Pruneda-Paz JL, Kim D, Kay SA, Walhout AJ, Ware D, Brady SM. (2011). Enhanced Y1H assays for Arabidopsis. Systems Biology Publications. https://doi.org/10.1038/nmeth.1750. Retrieved from https://escholarship.umassmed.edu/sysbio_pubs/10