2,3,7,8-Tetrachlorodibenzo-p-dioxin inhibits differentiation of normal diploid rat osteoblasts in vitro
Department of Cell Biology
Animals; Biological Markers; Cell Differentiation; Cells, Cultured; *Diploidy; Osteoblasts; Rats; Reference Values; Tetrachlorodibenzodioxin
The influence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a potent halogenated aromatic hydrocarbon, on the development of bone tissue-like organization in primary cultures of normal diploid calvarial-derived rat osteoblasts was examined. Initially, when placed in culture, these cells actively proliferate while expressing genes associated with biosynthesis of the bone extracellular matrix. Then, post-proliferatively, genes are expressed that render the osteoblast competent for extracellular matrix mineralization and maintenance of structural as well as functional properties of the mature bone-cell phenotype. Our results indicate that, in the presence of TCDD, proliferation of osteoblasts was not inhibited but post-confluent formation of multicellular nodules that develop bone tissue-like organization was dramatically suppressed. Consistent with TCDD-mediated abrogation of bone nodule formation, expression of alkaline phosphatase and osteocalcin was not upregulated post-proliferatively. These findings are discussed within the context of TCDD effects on estrogens and vitamin D-responsive developmental gene expression during osteoblast differentiation and, from a broader biological perspective, on steroid hormone control of differentiation.
DOI of Published Version
J Cell Biochem. 1994 Feb;54(2):231-8. Link to article on publisher's site
Journal of cellular biochemistry
Gierthy, John F.; Silkworth, J. B.; Tassinari, Melissa S.; Stein, Gary S.; and Lian, Jane B., "2,3,7,8-Tetrachlorodibenzo-p-dioxin inhibits differentiation of normal diploid rat osteoblasts in vitro" (1994). Stein, Stein, Lian, vanWijnen Lab Publications. 94.