Subnuclear localization and intranuclear trafficking of transcription factors
Department of Cell Biology
Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
DOI of Published Version
Methods Mol Biol. 2010;647:77-93. Link to article on publisher's site
Methods in molecular biology (Clifton, N.J.)
Zaidi, Sayyed K.; Medina, Ricardo F.; Pockwinse, Shirwin M.; Bakshi, Rachit; Kota, Krishna P.; Ali, Syed A.; Young, Daniel W.; Nickerson, Jeffrey A.; Javed, Amjad; Montecino, Martin A.; Van Wijnen, Andre J.; Lian, Jane B.; Stein, Janet L.; and Stein, Gary S., "Subnuclear localization and intranuclear trafficking of transcription factors" (2010). Stein, Stein, Lian, vanWijnen Lab Publications. 8.