Title

The histone gene transcription factor HiNF-P stabilizes its cell cycle regulatory co-activator p220NPAT

UMMS Affiliation

Department of Cell Biology

Publication Date

2006-12-21

Document Type

Article

Subjects

*Cell Cycle; Cell Cycle Proteins; Cyclin E; Cyclin-Dependent Kinase 2; Half-Life; Hela Cells; Histones; Humans; Nuclear Proteins; Proteasome Endopeptidase Complex; RNA, Messenger; Repressor Proteins; Signal Transduction; Trans-Activators; Ubiquitin-Protein Ligase Complexes

Disciplines

Cell Biology

Abstract

Orderly progression through the cell cycle requires the transcriptional activation of histone genes to support packaging of newly replicated DNA. Induction of human histone gene expression is mediated by a co-activation complex containing transcription factor HiNF-P and its cofactor p220NPAT. Here, using cells synchronized in S-phase and in mitosis, as well as serum-stimulated cells, we have investigated how HiNF-P is regulated during the cell cycle and examined its stability relative to p220NPAT. We find that while HiNF-P is maintained at steady-state levels throughout the cell cycle, both HiNF-P and p220NPAT are actively degraded by the proteasome pathway. Importantly, elevation of HiNF-P levels enhances the stability of its co-activator p220NPAT. The HiNF-P-dependent stabilization of p220NPAT may reinforce signaling through the cyclin E/CDK2/p220NPAT pathway and contribute to coordinate control of histone gene expression.

DOI of Published Version

10.1021/bi061425m

Source

Biochemistry. 2006 Dec 26;45(51):15915-20. Link to article on publisher's site

Journal/Book/Conference Title

Biochemistry

Related Resources

Link to Article in PubMed

PubMed ID

17176114

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