Title
Pluripotency: toward a gold standard for human ES and iPS cells
UMMS Affiliation
Department of Cell Biology
Publication Date
2009-07-28
Document Type
Article
Subjects
Adult Stem Cells; Biological Assay; *Cell Differentiation; *Cell Lineage; *Embryo Research; Embryonic Stem Cells; Gene Expression Regulation, Developmental; Humans; *Nuclear Reprogramming; Pluripotent Stem Cells; Reproducibility of Results; Terminology as Topic
Disciplines
Cell Biology
Abstract
With the advent of technologies for the derivation of embryonic stem cells and reprogrammed stem cells, use of the term "pluripotent" has become widespread. Despite its increased scientific and political importance, there are ambiguities with this designation and a common standard for experimental approaches that precisely define this state in human cells remains elusive. Recent studies have revealed that reprogramming may occur via many pathways which do not always lead to pluripotency. In addition, the pluripotent state itself appears to be highly dynamic, leading to significant variability in the results of molecular studies. Establishment of a stringent set of criteria for defining pluripotency will be vital for biological studies and potential clinical applications in this rapidly evolving field. In this review, we explore the various definitions of pluripotency, examine the current status of pluripotency testing in the field and provide an analysis of how these assays have been used to establish pluripotency in the scientific literature.
DOI of Published Version
10.1002/jcp.21681
Source
J Cell Physiol. 2009 Jul;220(1):21-9. Link to article on publisher's site
Journal/Book/Conference Title
Journal of cellular physiology
Related Resources
PubMed ID
19326392
Repository Citation
Smith, Kelly P.; Luong, Mai X.; and Stein, Gary S., "Pluripotency: toward a gold standard for human ES and iPS cells" (2009). Stein, Stein, Lian, vanWijnen Lab Publications. 38.
https://escholarship.umassmed.edu/stein/38