Title
Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells
UMMS Affiliation
Department of Cell Biology
Publication Date
2011-03-01
Document Type
Article
Subjects
Induced Pluripotent Stem Cells; Cell Cycle; G1 Phase
Disciplines
Cell Biology
Abstract
Induced pluripotent stem (iPS) cells derived from terminally differentiated human fibroblasts are re-programmed to possess stem cell like properties. However, the extent to which iPS cells exhibit unique properties of the human embryonic stem (hES) cell cycle remains to be established. Human ES cells are characterized by an abbreviated G1 phase ( approximately 2.5 h) and accelerated organization of subnuclear domains that mediate the assembly of regulatory machinery for histone gene expression [i.e., histone locus bodies (HLBs)]. We therefore examined cell cycle parameters of iPS cells in comparison to hES cells. Analysis of DNA synthesis (BrdU incorporation), cell cycle distribution (FACS analysis and Ki67 staining) and subnuclear organization of HLBs [immuno-fluorescence microscopy and fluorescence in situ hybridization (FISH)] revealed that human iPS cells have a short G1 phase ( approximately 2.5 h) and an abbreviated cell cycle (16-18 h). Furthermore, HLBs are formed and reorganized rapidly after mitosis (within 0.5 to 1.5 h). Thus, reprogrammed iPS cells have cell cycle kinetics and dynamic subnuclear organization of regulatory machinery that are principal properties of pluripotent hES cells. Our findings support the concept that the abbreviated cell cycle of hES and iPS cells is functionally linked to pluripotency. (c) 2010 Wiley-Liss, Inc.
DOI of Published Version
10.1002/jcp.22440
Source
J Cell Physiol. 2011 May;226(5):1149-56. doi: 10.1002/jcp.22440. Epub 2010 Oct 13. Link to article on publisher's site
Journal/Book/Conference Title
Journal of cellular physiology
Related Resources
PubMed ID
20945438
Repository Citation
Ghule, Prachi N.; Medina, Ricardo F.; Lengner, Christopher Joachim; Mandeville, Matthew; Qiao, Meng; Dominski, Zbigniew; Lian, Jane B.; Stein, Janet L.; Stein, Gary S.; and Van Wijnen, Andre J., "Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells" (2011). Stein, Stein, Lian, vanWijnen Lab Publications. 3.
https://escholarship.umassmed.edu/stein/3