Title
Osteoarthritic tissues modulate functional properties of sensory neurons associated with symptomatic OA pain
UMMS Affiliation
Department of Cell Biology
Publication Date
2011-11-18
Document Type
Article
Subjects
Animals; Coculture Techniques; Ganglia, Spinal; Gene Expression Regulation; Humans; Osteoarthritis; Pain; Rats; Rats, Sprague-Dawley; Sensory Receptor Cells; Synovial Membrane
Disciplines
Cell Biology
Abstract
Osteoarthritis (OA) is an age-related degenerative disease of cartilaginous tissues that is accompanied by hyperalgesia. Molecular cause and effect relationships between OA and pain remain to be elucidated. In this study, we have developed an experimental ex vivo organ co-culture system with dorsal root ganglia (DRGs) and knee synovial tissues from OA patients or unaffected human subjects. Our results suggest that tissues may generate symptomatic pain by altering the functional properties of sensory neurons. Specifically, we find that the expression levels of genes associated with neuronal pathways (e.g., SP, NK1, NK2, NPYR1, NPYR2, alpha2delta1) or inflammation (COX2/PTGS2 and IL6/interferon beta2) are clearly elevated in DRG explants cultured in the presence of OA derived synovial tissues. These findings are consistent with a model in which cytokines and pain molecules produced by knee synovium sensitize nociceptive neurons in tissues peripheral to joint cartilage.
DOI of Published Version
10.1007/s11033-011-0684-7
Source
Mol Biol Rep. 2011 Nov;38(8):5335-9. Epub 2011 Feb 16. Link to article on publisher's site
Journal/Book/Conference Title
Molecular biology reports
Related Resources
PubMed ID
21327824
Repository Citation
Li, Xin; Kim, Jae-Sung; Van Wijnen, Andre J.; and Im, Hee-Jeong, "Osteoarthritic tissues modulate functional properties of sensory neurons associated with symptomatic OA pain" (2011). Stein, Stein, Lian, vanWijnen Lab Publications. 262.
https://escholarship.umassmed.edu/stein/262