Heparan Sulfate Enhances the Self-Renewal and Therapeutic Potential of Mesenchymal Stem Cells from Human Adult Bone Marrow
Authors
Helledie, TorbenDombrowski, Christian
Rai, Bina
Lim, Zophia X.H.
Hin, Ian Lee Hock
Rider, David A.
Stein, Gary S.
Hong, Wanjin
Van Wijnen, Andre J.
Hui, James H.
Nurcombe, Victor
Cool, Simon M.
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2012-01-18
Metadata
Show full item recordAbstract
Insufficient cell number hampers therapies utilizing adult human mesenchymal stem cells (hMSCs) and current ex vivo expansion strategies lead to a loss of multipotentiality. Here we show that supplementation with an embryonic form of heparan sulfate (HS-2) can both increase the initial recovery of hMSCs from bone marrow aspirates and increase their ex vivo expansion by up to 13-fold. HS-2 acts to amplify a subpopulation of hMSCs harboring longer telomeres and increased expression of the MSC surface marker stromal precursor antigen-1. Gene expression profiling revealed that hMSCs cultured in HS-2 possess a distinct signature that reflects their enhanced multipotentiality and improved bone-forming ability when transplanted into critical-sized bone defects. Thus, HS-2 offers a novel means for decreasing the expansion time necessary for obtaining therapeutic numbers of multipotent hMSCs without the addition of exogenous growth factors that compromise stem cell fate.Source
Stem Cells Dev. 2012 Jan 18. Link to article on publisher's siteDOI
10.1089/scd.2011.0367Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49576PubMed ID
22066689Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1089/scd.2011.0367